LOCUS       sequence-159146-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop 
            codon (for native or N-terminal fusions), T35S mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Esposito
  TITLE     RAS mutant collection V2.0
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Hs.KRAS4b T35S
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..118
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             121..687
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPSIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     protein_bind    complement(686..710)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agctcggtcc gaggatccga aataatgatt ttattttgac tgatagtgac ctgttcgttg
       61 caacaaattg ataagcaatg cttttttata atgccaactt tgtacaaaaa agttggcacc
      121 atgactgaat ataaacttgt ggtagttgga gctggtggcg taggcaagag tgccttgacg
      181 atacagctaa ttcagaatca ttttgtggac gaatatgatc catcaataga ggattcctac
      241 aggaagcaag tagtaattga tggagaaacc tgtctcttgg atattctcga cacagcaggt
      301 caagaggagt acagtgcaat gagggaccag tacatgagga ctggggaggg ctttctttgt
      361 gtatttgcca taaataatac taaatcattt gaagatattc accattatag agaacaaatt
      421 aaaagagtta aggactctga agatgtacct atggtcctag taggaaataa atgtgatttg
      481 ccttctagaa cagtagacac aaaacaggct caggacttag caagaagtta tggaattcct
      541 tttattgaaa catcagcaaa gacaagacag ggtgttgatg atgccttcta tacattagtt
      601 cgagaaattc gaaaacataa agaaaagatg agcaaagatg gtaaaaagaa gaaaaagaag
      661 tcaaagacaa agtgtgtaat tatgtaatca actttcttgt acaaagttgg cattatagga
      721 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaat
//
