LOCUS       sequence-159147-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  High copy number plasmid containing hs671 MiniPromoter insert..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS1953
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(98..115)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcactgac tgactgactg cgtctcaacc tacgcgtgct gagattgtgt gatgtggaga
       61 aaaggaagat atgcagagat gttagcatgg ccacagggtg atggtggtgg tggtgctggg
      121 gcaagtgtgc actactaata ggagaagatg tcaaaaaaaa tgaagcataa taatagtgca
      181 atttatcctt gctaaacgta ctgcagtgtg ctataattaa atatgaaaac ctaaatttat
      241 ctctatgttc ggttgtcatt aatttaacag ggagtgcaca tgttctaatc taatggttga
      301 ttttttttct ccatgtgcag catattaaaa tgtatattag cctcaaggtg cataaacaca
      361 aaataataga ccaaaggttt tggcctgtgg agaaaaaaaa tccatgctca aaggccgtca
      421 agtagctgtg atgcagggga gacactcact tagcctcatt aagccaaagc tctcttttgc
      481 ttgtcttttg ttctgcatga cagcagcctc tttgttgtat aataattcat tccaagttcg
      541 ttccaagcta ccgaaactgg tgaactgtgg ccattttaat gagaagtatt ggtggtatgc
      601 ataatctgga agccacgtgg gccagacaat cacagagcac tgcagccagc aaaatgagat
      661 attgtcgaaa tcacaaattt gtcaagttgt ttttggacag ggtattataa taagcactaa
      721 aatatgcatc ctattactct gcatctgcca gtcaaagatc actgtaatga atgcgtgctg
      781 cagggtataa ccaggccgtt ttcctaagta aaacaaacca cagagtacag aaaagaattg
      841 tgtgggtttt tttttttttt tttggcattg tttgactttt ttttttttcc taatcctttt
      901 gcaccaggag tcaggttc
//
