LOCUS       sequence-159148-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  High copy number plasmid containing hs671 MiniPromoter insert..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS1953
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      229..238
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagcccttcc tctttccagt cagtcagtca gtcgtctctt gctacggcac tgcgcttggt
       61 ttagttgtca agagagaata actctcggac gcggaaggat tcgtagcgta tatcgcctat
      121 tgcggaagcg taaccaagtc ttacgacccc aaaagaggtt gactgaactg ggacgcggca
      181 ctggcgcaga tcacactgtc cgcgataaaa tcgggtgggt catggcgcgc cgccatggcg
      241 ccgcgctctg cttctggaag gctgcgctcc gcggcgtgga tgctccgggg aaagttgcgc
      301 tccgcggcag ggatgctcct gggaaggttg cgctccgcgg cagggatgct ctggggaagg
      361 ctggtcctgg ccgaggatcg ggaacgcgcc gctcgctctg cttctcttgt cttcgcttgt
      421 ctctggatgg aaccagattt ggttctgagt agctgtcagc gtctggtgac ctgctcgccg
      481 ccctgcgcct ttaaggagtc ttcaccggcc ccgcccactc tccgctgggc caatcagcga
      541 gccggaggag gccttggggc caggaatctt ccagcagttt cgcgtctggt ggagcttccc
      601 cgcctccctt gagtaatcgg agttgtgggt tccgcccttg tccagaactc tccagaggtt
      661 tctggggttc actggagagt acggattcct gagggggagg gtgtggggaa gtgctggtgc
      721 tactagtgac actgttgcta tggcgacgca ttactaaggc ctgtgtggaa tggacaagaa
      781 agatcacctc tagctcggtg ttgtgtacag tttgttgtga tttgtggggt ttcgccaact
      841 cgcacagttc tgaatatggg gg
//
