LOCUS       sequence-159151-         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  High copy number plasmid containing hs1218 MiniPromoter insert..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS1957
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      213..222
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagtcagtca gtcagtcgtc tcttggaacg gcactgcgct tggtttagtt gtcaagagag
       61 aataactctc ggacgcggaa ggattcgtag cgtatatcgc ctattgcgga agcgtaacca
      121 agtcttacga ccccaaaaga ggttgactga actgggacgc ggcactggcg cagatcacac
      181 tgtccgcgat aaaatcgggt gggtcatggc gcgccgccat ggcgccgcgc tctgcttctg
      241 gaaggctgcg ctccgcggcg tggatgctcc ggggaaagtt gcgctccgcg gcagggatgc
      301 tcctgggaag gttgcgctcc gcggcaggga tgctctgggg aaggctggtc ctggccgagg
      361 atcgggaacg cgccgctcgc tctgcttctc ttgtcttcgc ttgtctctgg atggaaccag
      421 atttggttct gagtagctgt cagcgtctgg tgacctgctc gccgccctgc gcctttaagg
      481 agtcttcacc ggccccgccc actctccgct gggccaatca gcgagccgga ggaggccttg
      541 gggccaggaa tcttccagca gtttcgcgtc tggtggagct tccccgcctc ccttgagtaa
      601 tcggagttgt gggttccgcc cttgtccaga actctccaga ggtttctggg gttcactgga
      661 gagtacggat tcctgagggg gagggtg
//
