LOCUS       sequence-159167-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ssAAV genome with Ple254 (PAX6 MiniPromoter) driving an emerald GFP 
            (EmGFP) reporter. Contains WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Hickmott JW, Chen CY, Arenillas DJ, Korecki AJ, Lam SL, Molday LL, 
            Bonaguro RJ, Zhou M, Chou AY, Mathelier A, Boye SL, Hauswirth WW, 
            Molday RS, Wasserman WW, Simpson EM
  TITLE     PAX6 MiniPromoters drive restricted expression from rAAV in the 
            adult mouse retina.
  JOURNAL   Mol Ther Methods Clin Dev. 2016 Aug 10;3:16051. doi: 
            10.1038/mtm.2016.51. eCollection 2016.
  PUBMED    27556059
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2016-08-10"; volume: "3:16051. doi"; 
            pages: " 10.1038/mtm.2016.51. eCollection 2016"
COMMENT     Sequence Label: pEMS2043
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(47..66)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          49..181
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcgg ccgcctggga gtggacacct gtggagagaa
       61 aggcaaagtg gatgtcagta agaccaatag gtgcctatca gaaacgcaag agtcttctct
      121 gtctcgacaa gcccagtttc tattggtctc cttaaacctg tcttgtaacc ttgatactta
      181 cctgcccagt gcctcacgac caacttcggc gcgcctgaca gccgcgttct acgcgaggac
      241 ctgccccaga gtttaaatgt caatgataag aaaagagggt gctcaggcag gcgctaactt
      301 tccttaatat ccacgccagc gccgtcctca ttggctgccc ggcccgcgtg acgtcatggc
      361 ggctagagtt gggcacagct ctgcgccgac tagttttccg gccgggcggg agcctgcttc
      421 tccccaccca gggtccggcc ggctccaacc cctgctttgg ccttccttgg cccggcctgt
      481 gactgctcta gcccggcccg gtccagcctg ggccgccgtc ctgtatggcg tagagtcgat
      541 ccccaggaaa ctagtcttgc cgagtgcgct cctccgcacc ttcgggcact agggccccaa
      601 gatctctgcc cttaggctgg ccagctacct ctctgggtaa tcaccctgtc ccctcctccg
      661 atccccaaga tcgggcagcc cagtccaagc aaccgtgccc ctgcccactg tactgcagca
      721 gcaacacaga gtcagacaca tgccaaggaa caagtacaca tggacctcac tacagccata
      781 accccagcag tgtccccagc ccactgcccc tcttcataaa cactgcagcc agcctctgaa
      841 cccacaaagc gctaagaacc ggctctataa atctcccaaa gatcccctct gagtgtcacc
      901 gcaaaccatg ggttctacag atcccacact gaacc
//
