LOCUS       sequence-159176-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ssAAV genome with Ple258 (PAX6 MiniPromoter) driving an emerald GFP 
            (EmGFP) reporter. Contains WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Hickmott JW, Chen CY, Arenillas DJ, Korecki AJ, Lam SL, Molday LL, 
            Bonaguro RJ, Zhou M, Chou AY, Mathelier A, Boye SL, Hauswirth WW, 
            Molday RS, Wasserman WW, Simpson EM
  TITLE     PAX6 MiniPromoters drive restricted expression from rAAV in the 
            adult mouse retina.
  JOURNAL   Mol Ther Methods Clin Dev. 2016 Aug 10;3:16051. doi: 
            10.1038/mtm.2016.51. eCollection 2016.
  PUBMED    27556059
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2016-08-10"; volume: "3:16051. doi"; 
            pages: " 10.1038/mtm.2016.51. eCollection 2016"
COMMENT     Sequence Label: pEMS2047
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(43..62)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          45..177
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcggccgc ctgggagtgg acacctgtgg agagaaaggc
       61 aaagtggatg tcagtaagac caataggtgc ctatcagaaa cgcaagagtc ttctctgtct
      121 cgacaagccc agtttctatt ggtctcctta aacctgtctt gtaaccttga tacttacctg
      181 cccagtgcct cacgaccaac ttcggcgcgc ctgacagccg cgttctacgc gaggacctgc
      241 cccagagttt aaatgtcaat gataagaaaa gagggtgctc aggcaggcgc taactttcct
      301 taatatccac gccagcgccg tcctcattgg ctgcccggcc cgcgtgacgt catggcggct
      361 agagttgggc acagctctgc gccgactagt tttccggccg ggcgggagcc tgcttctccc
      421 cacccagggt ccggccggct ccaacccctg ctttggcctt ccttggcccg gcctgtgact
      481 gctctagccc ggcccggtcc agcctgggcc gccgtcctgt atggcgtaga gtcgatcccc
      541 aggaaactag tcttgccgag tgcgctcctc cgcaccttcg ggcactaggg ccccaagatc
      601 tctgccctta ggctggccag ctacctctct gggtaatcac cctgtcccct cctccgatcc
      661 ccaagtaaga ttaaatgaaa gaagtaaatc ctctttgaaa taagcaaatg tcaggttgac
      721 catttaaata cacagtactt tgcttatgta ctatcagaat ggaaatttat ctcatcttta
      781 aaccttgcaa tttctgttat tcatataatt gagaaagtcc tagtttatat cactgaaatc
      841 ttgaatagat gagttctaac aaaaattgac ttgtatcatt ttagtttcag atatttagat
      901 aaaaaggctt agtcttttat ttatagataa a
//
