LOCUS       sequence-159179-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ssAAV genome with Ple259 (PAX6 MiniPromoter) driving an emerald GFP 
            (EmGFP) reporter. Contains WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Hickmott JW, Chen CY, Arenillas DJ, Korecki AJ, Lam SL, Molday LL, 
            Bonaguro RJ, Zhou M, Chou AY, Mathelier A, Boye SL, Hauswirth WW, 
            Molday RS, Wasserman WW, Simpson EM
  TITLE     PAX6 MiniPromoters drive restricted expression from rAAV in the 
            adult mouse retina.
  JOURNAL   Mol Ther Methods Clin Dev. 2016 Aug 10;3:16051. doi: 
            10.1038/mtm.2016.51. eCollection 2016.
  PUBMED    27556059
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2016-08-10"; volume: "3:16051. doi"; 
            pages: " 10.1038/mtm.2016.51. eCollection 2016"
COMMENT     Sequence Label: pEMS2048
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(39..58)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          41..173
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 cccttgctca ccatggtggc ggccgcctgg gagtggacac ctgtggagag aaaggcaaag
       61 tggatgtcag taagaccaat aggtgcctat cagaaacgca agagtcttct ctgtctcgac
      121 aagcccagtt tctattggtc tccttaaacc tgtcttgtaa ccttgatact tacctgccca
      181 gtgcctcacg accaacttcg gcgcgcctga cagccgcgtt ctacgcgagg acctgcccca
      241 gagtttaaat gtcaatgata agaaaagagg gtgctcaggc aggcgctaac tttccttaat
      301 atccacgcca gcgccgtcct cattggctgc ccggcccgcg tgacgtcatg gcggctagag
      361 ttgggcacag ctctgcgccg actagttttc cggccgggcg ggagcctgct tctccccacc
      421 cagggtccgg ccggctccaa cccctgcttt ggccttcctt ggcccggcct gtgactgctc
      481 tagcccggcc cggtccagcc tgggccgccg tcctgtatgg cgtagagtcg atccccagga
      541 aactagtctt gccgagtgcg ctcctccgca ccttcgggca ctagggcccc aagatctctg
      601 cccttaggct ggccagctac ctctctgggt aatcaccctg tcccctcctc cgatccccaa
      661 gaggcctcct cagtgcgcag gcgccgttcg ggcccgccgc cccctcccaa ggcgtggggt
      721 cccggggaca gaccccgagc ccctctctgc gcaactaccg cctctaaaaa aaagaccatc
      781 tggtaattgt ctttctttag atcttccctt aattatgctt ttaattaaag atggttcaag
      841 gcaccggacc acatgaaggg cagtaattac aaattacatt acaaac
//
