LOCUS       sequence-159181-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ssAAV genome with Ple260 (PAX6 MiniPromoter) driving an emerald GFP 
            (EmGFP) reporter. Contains WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Hickmott JW, Chen CY, Arenillas DJ, Korecki AJ, Lam SL, Molday LL, 
            Bonaguro RJ, Zhou M, Chou AY, Mathelier A, Boye SL, Hauswirth WW, 
            Molday RS, Wasserman WW, Simpson EM
  TITLE     PAX6 MiniPromoters drive restricted expression from rAAV in the 
            adult mouse retina.
  JOURNAL   Mol Ther Methods Clin Dev. 2016 Aug 10;3:16051. doi: 
            10.1038/mtm.2016.51. eCollection 2016.
  PUBMED    27556059
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2016-08-10"; volume: "3:16051. doi"; 
            pages: " 10.1038/mtm.2016.51. eCollection 2016"
COMMENT     Sequence Label: pEMS2049
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(39..58)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          41..173
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 cccttgctca ccatggtggc ggccgcctgg gagtggacac ctgtggagag aaaggcaaag
       61 tggatgtcag taagaccaat aggtgcctat cagaaacgca agagtcttct ctgtctcgac
      121 aagcccagtt tctattggtc tccttaaacc tgtcttgtaa ccttgatact tacctgccca
      181 gtgcctcacg accaacttcg gcgcgccatc ctccagcaaa acacttcctc ctgcgcctga
      241 accagagcgg gaaatgaggc cgagccacgg ttcccttttc aaacccacta atcactccgc
      301 aacatgcaaa tgcaccgctc gctctcacac aaaatattgc tttatgcaaa gcagcgccgg
      361 ggcctcgcgc cagccgattg gatgctccct ctccccgcct ggtgcaattg gtccgtttgt
      421 ttgaatatga atacacttgt tttcggctcc gccggtgggt cccggctcct ctccccggcg
      481 cagggccccc gagcccgggc tctggcgctc gcctgcatct ccccgctccg cgcacgccga
      541 gattcggcgc ggcccgccct gctgccgcga acttgagcgg tcaagtgaag gtttctgggt
      601 tgggggtcgc gcccccactc cccctaagct ggactcggga ctcccagtcc tcggagggtg
      661 atcgggcagc ccagtccaag caaccgtgcc cctgcccact gtactgcagc agcaacacag
      721 agtcagacac atgccaagga acaagtacac atggacctca ctacagccat aaccccagca
      781 gtgtccccag cccactgccc ctcttcataa acactgca
//
