LOCUS       sequence-159183-        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of STK3. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: STK3_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             55..77
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             97..126
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             127..147
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     RBS             762..770
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggagagctct ttaattaagc ggccgccctg caggactcga gttctagaaa taattttgtt
       61 taactttaag aaggagatat acatatgaaa tcttctcacc atcaccatca ccatcaccat
      121 caccatgaaa acctgtactt ccaatccaat gcagaagaca gtttgactaa gcagcctgaa
      181 gaagtttttg atgtattaga gaagcttgga gaagggtctt atggaagtgt atttaaagca
      241 atacacaagg aatccggtca agttgtcgca attaaacaag tacctgttga atcagatctt
      301 caggaaataa tcaaagaaat ttccataatg cagcaatgtg acagcccata tgttgtaaag
      361 tactatggca gttattttaa gaatacagac ctctggattg ttatggagta ctgtggcgct
      421 ggctctgtct cagacataat tagattacga aacaagacat taatagaaga tgaaattgca
      481 accattctta aatctacatt gaaaggacta gaatatttgc actttatgag aaaaatacac
      541 agagatataa aagctggaaa tattctcctc aatacagaag gacatgcaaa attggcagat
      601 tttggagtgg ctggtcagtt aacagataca atggcaaaac gcaatactgt aataggaact
      661 ccattttgga tggctcctga ggtgattcaa gaaataggct ataactgtgt ggccgacatc
      721 tggtcccttg gcattacttc tatagaaatg gctgaaggaa aacctcctta tgctgatata
      781 catccaatga gggctatttt tatgattccc acaaatccac caccaacatt cagaaagcca
      841 gaactttggt ccgatgattt caccgatttt gttaaaaagt gtttggtgaa gaatcctgag
      901 cagagagcta ctgcaacaca acttttacag catcctttta tcaagaatgc caaacctgta
      961 tcaatattaa gagacctgat cacagaagct atggagatca aagctaaaag acatgaggaa
     1021 cagc
//
