LOCUS       sequence-159184-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of STK24. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: STK24_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(926..946)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(947..976)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatcagcg cgcacgcgtg gtaccggccg gccaccaggt
       61 ggcgcgccgc gatcgcggat ccgttatcca cttccaatgt tattagtcga tgagctcggt
      121 caagtaggaa gttttctttg cattgcgtag tataaacttg tgcttcaata actccttagc
      181 agtgggtcta aagctcggct ccttattcaa acaggcctcc acaaactcct tgaggggttt
      241 actgtagttt ccttccaacg tcggtgggtt gttctttgga atgaggaata aaactttcat
      301 ggggtgcagc tcggaatgag gtggttcccc tcttgcaagt tcaatagctg ttatgcccag
      361 ggaccagatg tctgccttcg agtcataggc cgactgtttg atgacctcgg gtgccatcca
      421 gaatggggtg cccacgaagg tgttcctttt gatctgggtg tctgtcagct ggccagccac
      481 gccaaagtcc gccagcttca cctcgccatg ctcagacagc aggacgttgg ccgctttaat
      541 gtctctgtgg attttcttct ccgaatggag ataatcgagt cctttcagta tttctcttaa
      601 tatagtagcg atctgggttt catctaatgg gccaggttct aatagatcta gtgcggagcc
      661 tccaccaaga tattccatta ttatccataa ttttgtatcc ttcagatagg atccataata
      721 tttggttaca tatggactgt cacactgact cagcactgtg atttcttgtt gaatgtcctc
      781 tatctcatct tcagcttctt ccagatcaat gatctttatg gcaaccactt tctgagtccg
      841 attgtcaatg cctttgaaca cctctccaaa ggagcccttc ccaattttct ctagttttgt
      901 aaaaagctct tctggggtct gcattggatt ggaagtacag gttttcatgg tgatggtgat
      961 ggtgatggtg atggtgagaa gatttcatat g
//
