LOCUS       sequence-159185-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of STK24. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: STK24_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             58..80
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..129
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             130..150
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tccggagagc tctttaatta agcggccgcc ctgcaggact cgagttctag aaataatttt
       61 gtttaacttt aagaaggaga tatacatatg aaatcttctc accatcacca tcaccatcac
      121 catcaccatg aaaacctgta cttccaatcc aatgcagacc cagaagagct ttttacaaaa
      181 ctagagaaaa ttgggaaggg ctcctttgga gaggtgttca aaggcattga caatcggact
      241 cagaaagtgg ttgccataaa gatcattgat ctggaagaag ctgaagatga gatagaggac
      301 attcaacaag aaatcacagt gctgagtcag tgtgacagtc catatgtaac caaatattat
      361 ggatcctatc tgaaggatac aaaattatgg ataataatgg aatatcttgg tggaggctcc
      421 gcactagatc tattagaacc tggcccatta gatgaaaccc agatcgctac tatattaaga
      481 gaaatactga aaggactcga ttatctccat tcggagaaga aaatccacag agacattaaa
      541 gcggccaacg tcctgctgtc tgagcatggc gaggtgaagc tggcggactt tggcgtggct
      601 ggccagctga cagacaccca gatcaaaagg aacaccttcg tgggcacccc attctggatg
      661 gcacccgagg tcatcaaaca gtcggcctat gactcgaagg cagacatctg gtccctgggc
      721 ataacagcta ttgaacttgc aagaggggaa ccacctcatt ccgagctgca ccccatgaaa
      781 gttttattcc tcattccaaa gaacaaccca ccgacgttgg aaggaaacta cagtaaaccc
      841 ctcaaggagt ttgtggaggc ctgtttgaat aaggagccga gctttagacc cactgctaag
      901 gagttattga agcacaagtt tatactacgc aatgcaaaga aaacttccta cttgaccgag
      961 ctcatcgact aataacattg gaagtggata acggatccgc gatcgcggcg cgccacctgg
     1021 tggccggccg g
//
