LOCUS       sequence-159186-         603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  microRNA reporter relying on a bidirectional PGK promoter. Contains 
            MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA 
            binding sites. Contains a Hygromycin selection cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors 
            in Embryonic Stem Cells.
  JOURNAL   PLoS One. 2016 May 6;11(5):e0155177. doi: 
            10.1371/journal.pone.0155177. eCollection 2016.
  PUBMED    27152616
REFERENCE   2  (bases 1 to 603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-05-6"; volume: "11(5):e0155177. doi"; pages: " 
            10.1371/journal.pone.0155177. eCollection 2016"
COMMENT     Sequence Label: miReporter-PGK
FEATURES             Location/Qualifiers
     source          1..603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     213..234
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
       61 ggcgaagcac atcaggccgt agccgaaggt ggtcacgagg gtgggccagg gcacgggcag
      121 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      181 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      241 caccaccccg gtgaacagct cctcgccctt gctcaccatc tcgaccttag cgctggtgta
      301 cttggtgatg gccttagtac cctcggacac ggcgtgcttg gccaactccc caggcagcag
      361 caggcgcacg gccgtctgga tctccctgga ggtgatggtc gagcgcttgt tgtaatgcgc
      421 caggcgggaa gcctcacctg cgatgcgctc gaaaatgtcg ttcacaaacg aattcatgat
      481 gcccatggcc ttggacgaaa tgccggtgtc agggtggacc tgcttcagaa ccttgtacac
      541 atagatggaa tagctctcct tgcggctgcg cttgcgcttc ttgccgcctt tcttctgcgc
      601 ctt
//
