LOCUS       sequence-159187-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Livingston CM, Ifrim MF, Cowan AE, Weller SK
  TITLE     Virus-Induced Chaperone-Enriched (VICE) domains function as nuclear 
            protein quality control centers during HSV-1 infection.
  JOURNAL   PLoS Pathog. 2009 Oct . 5(10):e1000619.
  PUBMED    19816571
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog. 2009 Oct . 5(10):e1000619."
COMMENT     Sequence Label: pRK5-HA-Ubiquitin-K11
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             97..123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             154..381
                     /codon_start=1
                     /product="human ubiquitin variant containing Lys-11, with 
                     all other lysines mutated to arginine"
                     /label=ubiquitin-K11
                     /translation="MQIFVRTLTGKTITLEVEPSDTIENVRARIQDREGIPPDQQRLIF
                     AGRQLEDGRTLSDYNIQRESTLHLVLRLRGG"
     regulatory      418..427
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    431..565
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(468..487)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     522..541
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        634..825
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(657..677)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtccactccc aggtccaact gcacctcggt tctatcgatt gaattcccga accgacagtc
       61 ggtctcttca ccaaggccat tcgcgccacc atgggctacc cctatgatgt gcctgactac
      121 gcagatctca atggtggtgg tggtgggtcg accatgcaga tcttcgtcag aacgttaacc
      181 ggtaaaacca taactctaga agttgaacca tccgatacca tcgaaaacgt tagagctaga
      241 attcaagaca gagaaggcat tccacctgat caacaaagat tgatctttgc cggtagacag
      301 ctcgaggacg gtagaacgct gtctgattac aacattcaga gagagtcgac cttacatctt
      361 gtcttaagac taagaggtgg ttgagcggcc gctaagtaag taaggatccc cagcttggcc
      421 gccatggccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      481 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
      541 caatgtatct tatcatgtct ggatcgggaa ttaattcggc gcagcaccat ggcctgaaat
      601 aacctctgaa agaggaactt ggttaggtac cttctgaggc ggaaagaacc agctgtggaa
      661 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      721 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
      781 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
      841 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatgg
//
