LOCUS       sequence-159195-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of epitope-tagged human KLF12 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Godin-Heymann N, Brabetz S, Murillo MM, Saponaro M, Santos CR, 
            Lobley A, East P, Chakravarty P, Matthews N, Kelly G, Jordan S, 
            Castellano E, Downward J
  TITLE     Tumour-suppression function of KLF12 through regulation of anoikis.
  JOURNAL   Oncogene. 2016 Jun 23;35(25):3324-34. doi: 10.1038/onc.2015.394. 
            Epub 2015 Oct 12.
  PUBMED    26455320
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2015.394"; journalName: "Oncogene"; date: "2016-06-23- 
            23"; volume: "35"; issue: "25"; pages: "3324-34"
COMMENT     Sequence Label: FLAG-KLF12/pcDNA3.1-Neo
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             125..148
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg gaattcccac
      121 catggattac aaggatgacg atgacaagaa tatccatatg aagagaaaaa caataaagaa
      181 tatcaacacc tttgagaaca gaatgttaat gcttgatggg atgccggcag tcagagtcaa
      241 aacagagctt ttggaatctg aacaagggtc tccaaacgtc cacaactatc ccgatatgga
      301 agccgttccc ctgttgctaa ataatgtgaa aggggagccc ccggaggact cgttatctgt
      361 agatcacttc caaacacaaa ctgagccagt ggacttgtca ataaacaaag ccaggacgtc
      421 ccctactgcc gtttcatcct ccccagtttc catgacagca tctgcctcct caccttcttc
      481 aacttcaacc tcttcatcgt cttctagtcg tctagcctca tccccaactg ttatcacatc
      541 agtatcttca gcgtcatctt cgtcaacagt attaactcca gggccccttg tggcctctgc
      601 atctggtgtt ggaggccagc agtttttgca cattatccat cccgtaccgc cttcaagtcc
      661 catgaattta cagtctaaca aactgagtca tgttcaccgc atccccgtgg tggtacagtc
      721 ggtgcctgtt gtctacacag ctgtaaggtc acctggaaat gtgaacaaca ctattgtcgt
      781 gccgcttttg gaggatggga gaggccatgg caaagcacaa atggaccccc gaggcctatc
      841 tcccagacaa agtaaaagtg acagtgatga tgatgacct
//
