LOCUS       sequence-159197-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Fujita T, Kitaura F, Oji A, Tanigawa N, Yuno M, Ikawa M, Taniuchi I,
            Fujii H
  TITLE     Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for 
            enChIP analysis.
  JOURNAL   Genes Cells. 2018 Feb 26. doi: 10.1111/gtc.12573.
  PUBMED    29480524
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Cells. 2018 Feb 26. doi: 10.1111/gtc.12573."
COMMENT     Sequence Label: 3xFLAG-dCas9/MSCV-EGFP
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             98..163
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 tcctcccttt atccagccct cactccttct ctaggcgccg gaattagatc tctcgaggtt
       61 taaggatccg tttagtgaac cgtcagaatt aaccatggac tacaaagacc atgacggtga
      121 ttataaagat catgacatcg attacaagga tgacgatgac aagctcatgg acaagaagta
      181 ctccattggg ctcgctatcg gcacaaacag cgtcggctgg gccgtcatta cggacgagta
      241 caaggtgccg agcaaaaaat tcaaagttct gggcaatacc gatcgccaca gcataaagaa
      301 gaacctcatt ggcgccctcc tgttcgactc cggggagacg gccgaagcca cgcggctcaa
      361 aagaacagca cggcgcagat atacccgcag aaagaatcgg atctgctacc tgcaggagat
      421 ctttagtaat gagatggcta aggtggatga ctctttcttc cataggctgg aggagtcctt
      481 tttggtggag gaggataaaa agcacgagcg ccacccaatc tttggcaata tcgtggacga
      541 ggtggcgtac catgaaaagt acccaaccat atatcatctg aggaagaagc ttgtagacag
      601 tactgataag gctgacttgc ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg
      661 gggacacttc ctcatcgagg gggacctgaa cccagacaac agcgatgtcg acaaactctt
      721 tatccaactg gttcagactt acaatcagct tttcgaagag aacccgatca acgcatccgg
      781 agttgacgcc aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct
      841 catcgcacag ctccctgggg agaagaagaa cggcctgttt ggtaatctta tcgccctgtc
      901 actcgggctg acccccaact ttaaatctaa cttcgacctg g
//
