LOCUS       sequence-159206-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop 
            codon (for native or N-terminal fusions), R73K mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Esposito
  TITLE     RAS mutant collection V2.0
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Hs.KRAS4b R73K
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..123
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             126..692
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMKTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     protein_bind    complement(691..715)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
       61 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      121 gcaccatgac tgaatataaa cttgtggtag ttggagctgg tggcgtaggc aagagtgcct
      181 tgacgataca gctaattcag aatcattttg tggacgaata tgatccaaca atagaggatt
      241 cctacaggaa gcaagtagta attgatggag aaacctgtct cttggatatt ctcgacacag
      301 caggtcaaga ggagtacagt gcaatgaggg accagtacat gaagactggg gagggctttc
      361 tttgtgtatt tgccataaat aatactaaat catttgaaga tattcaccat tatagagaac
      421 aaattaaaag agttaaggac tctgaagatg tacctatggt cctagtagga aataaatgtg
      481 atttgccttc tagaacagta gacacaaaac aggctcagga cttagcaaga agttatggaa
      541 ttccttttat tgaaacatca gcaaagacaa gacagggtgt tgatgatgcc ttctatacat
      601 tagttcgaga aattcgaaaa cataaagaaa agatgagcaa agatggtaaa aagaagaaaa
      661 agaagtcaaa gacaaagtgt gtaattatgt aatcaacttt cttgtacaaa gttggcatta
      721 taggaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
//
