LOCUS       sequence-159207-        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of KCC2D. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: KCC2D_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             62..84
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             104..133
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             134..154
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cggctccgga gagctcttta attaagcggc cgccctgcag gactcgagtt ctagaaataa
       61 ttttgtttaa ctttaagaag gagatataca tatgaaatct tctcaccatc accatcacca
      121 tcaccatcac catgaaaacc tgtacttcca atccaatgca acggacgagt atcagctttt
      181 cgaggagctt ggaaaggggg cattctcagt ggtgagaaga tgtatgaaaa ttcctactgg
      241 acaagaatat gctgccaaaa ttatcaacac caaaaagctt tctgctaggg atcatcagaa
      301 actagaaaga gaagctagaa tctgccgtct tttgaagcac cctaatattg tgcgacttca
      361 tgatagcata tcagaagagg gctttcacta cttggtgttt gatttagtta ctggaggtga
      421 actgtttgaa gacatagtgg caagagaata ctacagtgaa gctgatgcca gtcattgtat
      481 acagcagatt ctagaaagtg ttaatcattg tcacctaaat ggcatagttc acagggacct
      541 gaagcctgag aatttgcttt tagctagcaa atccaaggga gcagctgtga aattggcaga
      601 ctttggctta gccatagaag ttcaagggga ccagcaggcg tggtttggtt ttgctggcac
      661 acctggatat ctttctccag aagttttacg taaagatcct tatggaaagc cagtggatat
      721 gtgggcatgt ggtgtcattc tctatattct acttgtgggg tatccaccct tctgggatga
      781 agaccaacac agactctatc agcagatcaa ggctggagct tatgattttc catcaccaga
      841 atgggacacg gtgactcctg aagccaaaga cctcatcaat aaaatgctta ctatcaaccc
      901 tgccaaacgc atcacagcct cagaggcact gaagcaccca tggatctgtc aacgttctac
      961 tgttgcttcc atgatgcaca gacaggagac tgtagactgc ttgaagaaat ttaatgctag
     1021 aagaaaacta aagggtgcca t
//
