LOCUS       sequence-159210-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses myc tag and catalytic inactive form of mRNA capping enzyme
            with N terminal Nuclear export signal (NES).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Otsuka Y, Kedersha NL, Schoenberg DR
  TITLE     Identification of a cytoplasmic complex that adds a cap onto 
            5'-monophosphate RNA.
  JOURNAL   Mol Cell Biol. 2009 Apr;29(8):2155-67. doi: 10.1128/MCB.01325-08. 
            Epub 2009 Feb 17.
  PUBMED    19223470
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01325-08"; journalName: "Mol Cell Biol"; date: 
            "2009-04"; volume: "29"; issue: "8"; pages: "2155-67"
COMMENT     Sequence Label: pcDNA3-myc NES- mCE(K294A)
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             158..184
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac caccatggca tcaatgcaga agctgatctc
      121 agaggaggac ctgcttatgg ccatggaggc ccttcagcta ccaccgcttg agagacttac
      181 tcttgatatg gccatggagg ccgcttacaa caagatcccg ccgcggtggc tgaactgtcc
      241 gcggcgcggt cagccggtgg caggaagatt cttacctctg aagacgatgc tggggccaag
      301 atatgatagt caagttgctg aagaaaaccg gttccatccc agtatgctct caaattatct
      361 aaagagcctg aaggttaaaa tgagcttgtt ggtggatctg acaaatactt caaggttcta
      421 tgacagaaat gacatagaaa aagaaggaat caagtatatc aaacttcagt gtaaaggaca
      481 tggcgaatgc cccaccactg agaatactga gacattcatt cgcctgtgtg agaggtttaa
      541 tgaaagaagc ccacctgaac ttataggtgt tcactgcacc catggcttca accgtactgg
      601 tttccttata tgtgcctttt tggtggaaaa aatggattgg agtattgaag cagcagttgc
      661 tacatttgcc caagccagac caccaggaat ctacaagggt gattatttga aggaactttt
      721 tcgtcgctat ggagatatag aagaagcacc accaccacct gtattgccag attggtgttt
      781 tgaagatgag gatgaagaag atgaggatga agatggaaaa aaggactcag aaccagggtc
      841 aagtgcatcc tttagtaaaa ggagaaaaga gcggttaaaa ctgggagcta ttttcttgga
      901 aggcataact gttaaaggtg taactcaagt aacaactcaa cccaagttag gagaggtgca
      961 gcagaa
//
