LOCUS       sequence-159212-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop 
            codon (for native or N-terminal fusions), G12D/C185S mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Esposito
  TITLE     RAS mutant collection V2.0
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Hs.KRAS4b G12D/C185S
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..124
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(127..159,160..162,163..693)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="MTEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKSVIM"
     CDS             127..693
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKSVIM"
     protein_bind    complement(692..791)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 aattcgagct cggtccgagg atccgaaata atgattttat tttgactgat agtgacctgt
       61 tcgttgcaac aaattgataa gcaatgcttt tttataatgc caactttgta caaaaaagtt
      121 ggcaccatga ctgaatataa acttgtggta gttggagctg atggcgtagg caagagtgcc
      181 ttgacgatac agctaattca gaatcatttt gtggacgaat atgatccaac aatagaggat
      241 tcctacagga agcaagtagt aattgatgga gaaacctgtc tcttggatat tctcgacaca
      301 gcaggtcaag aggagtacag tgcaatgagg gaccagtaca tgaggactgg ggagggcttt
      361 ctttgtgtat ttgccataaa taatactaaa tcatttgaag atattcacca ttatagagaa
      421 caaattaaaa gagttaagga ctctgaagat gtacctatgg tcctagtagg aaataaatgt
      481 gatttgcctt ctagaacagt agacacaaaa caggctcagg acttagcaag aagttatgga
      541 attcctttta ttgaaacatc agcaaagaca agacagggtg ttgatgatgc cttctataca
      601 ttagttcgag aaattcgaaa acataaagaa aagatgagca aagatggtaa aaagaagaaa
      661 aagaagtcaa agacaaagtc tgtaattatg taatcaactt tcttgtacaa agttggcatt
      721 ataggaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
      781 atcattattt gcatgcaagc ttagatcctc tagaccagcc aggacagaaa tgcctcgact
      841 tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
      901 cagtggacat aagcctgttc ggttcgtaag ctgta
//
