LOCUS       sequence-159226-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the dehydrogenase-deficient mutant 
            DLD-H450A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, 
            Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, 
            Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster
            LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson 
            J, Babu M
  TITLE     A Map of Human Mitochondrial Protein Interactions Linked to 
            Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and 
            NF-kappaB Signaling.
  JOURNAL   Cell Syst. 2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010.
  PUBMED    29128334
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010."
COMMENT     Sequence Label: pPHAGE_DLD-H450A-C-TAP
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(754..778)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             810..833
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             849..875
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             849..875
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aaaactttca acgcatcctt caaaaacagg ggtttaaatt taaattgaat acaaaggtta
       61 ctggtgctac caagaagtca gatggaaaaa ttgatgtttc tattgaagct gcttctggtg
      121 gtaaagctga agttatcact tgtgatgtac tcttggtttg cattggccga cgacccttta
      181 ctaagaattt gggactagaa gagctgggaa ttgaactaga tcccagaggt agaattccag
      241 tcaataccag atttcaaact aaaattccaa atatctatgc cattggtgat gtagttgctg
      301 gtccaatgct ggctcacaaa gcagaggatg aaggcattat ctgtgttgaa ggaatggctg
      361 gtggtgctgt gcacattgac tacaattgtg tgccatcagt gatttacaca caccctgaag
      421 ttgcttgggt tggcaaatca gaagagcagt tgaaagaaga gggtattgag tacaaagttg
      481 ggaaattccc atttgctgct aacagcagag ctaagacaaa tgctgacaca gatggcatgg
      541 tgaagatcct tgggcagaaa tcgacagaca gagtactggg agcacatatt cttggaccag
      601 gtgctggaga aatggtaaat gaagctgctc ttgctttgga atatggagca tcctgtgaag
      661 atatagctag agtctgtgca gcacatccga ccttatcaga agcttttaga gaagcaaatc
      721 ttgctgcgtc atttggcaaa tcaatcaact tttgcccaac tttcttgtac aaagtggttg
      781 atctagaggg cccgcggttc gaactcgagg actacaagga cgacgatgac aagtcggccg
      841 ctggaggata cccctacgac gtgcccgact acgcctagaa ttccgccccc cccccctaac
      901 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt t
//
