LOCUS       sequence-159227-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian 
            cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Perli SD, Cui CH, Lu TK
  TITLE     Continuous genetic recording with self-targeting CRISPR-Cas in human
            cells.
  JOURNAL   Science. 2016 Aug 18. pii: aag0511.
  PUBMED    27540006
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 18. pii: aag0511."
COMMENT     Sequence Label: Construct 7 - CMVp-Cas9-3xNLS-HSVpA
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaccgtcaga tccgagctca tcaccggtgc gctgccacca tggacaagaa gtacagcatc
       61 ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg
      121 cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg
      181 atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc
      241 gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc
      301 aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg
      361 gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc
      421 taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac
      481 aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac
      541 ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag
      601 ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac
      661 gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc
      721 cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct gagcctgggc
      781 ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc
      841 aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc
      901 gacctgt
//
