LOCUS       sequence-159229-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral construct for building stable cell lines expressing 
            mKate2 in the presence of TNFa via puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Perli SD, Cui CH, Lu TK
  TITLE     Continuous genetic recording with self-targeting CRISPR-Cas in human
            cells.
  JOURNAL   Science. 2016 Aug 18. pii: aag0511.
  PUBMED    27540006
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 18. pii: aag0511."
COMMENT     Sequence Label: Construct 32 - NFKBRp_mKate2_2xNLS_p2a-puroR
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      264..273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aggtccgagg ttctagacag gtgccagaac atttctctat cgataggtac cggggacttt
       61 ccgggaattt ccggggactt tccgggaatt tccgggaatt tccggggact ttccgggaat
      121 ttccggggac tttccgggaa tttccagatc tggcctcggc ggccaagctt gctagcgggg
      181 ggctataaaa gggggtgggg gcgttcgtcc tcactctagt tctgcgatct aagtaagctt
      241 ggcattaccg gtcgccaacg cgtgccacca tggtgagcga gctgattaag gagaacatgc
      301 acatgaagct gtacatggag ggcaccgtga acaaccacca cttcaagtgc acatccgagg
      361 gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggcggtc gagggcggcc
      421 ctctcccctt cgccttcgac atcctggcta ccagcttcat gtacggcagc aaaaccttca
      481 tcaaccacac ccagggcatc cccgacttct ttaagcagtc cttccccgag ggcttcacat
      541 gggagagagt caccacatac gaagatgggg gcgtgctgac cgctacccag gacaccagcc
      601 tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc ccatccaacg
      661 gccctgtgat gcagaagaaa acactcggct gggaggcctc caccgagaca ctgtaccccg
      721 ctgacggcgg cctggaaggc agagccgaca tggccctgaa gctcgtgggc gggggccacc
      781 tgatctgcaa ccttaagacc acatacagat ccaagaaacc cgctaagaac ctcaagatgc
      841 ccggcgtcta ctatgtggac aggagactgg aaa
//
