LOCUS       sequence-159230-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expressing Dox and IPTG inducible stgRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Perli SD, Cui CH, Lu TK
  TITLE     Continuous genetic recording with self-targeting CRISPR-Cas in human
            cells.
  JOURNAL   Science. 2016 Aug 18. pii: aag0511.
  PUBMED    27540006
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 18. pii: aag0511."
COMMENT     Sequence Label: Construct 31 -
            1xTetO_H1p_20nt2_stgRNA_3xLacO_...
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..21
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     233..253
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     protein_bind    342..358
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     404..423
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    435..454
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    596..615
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     primer_bind     complement(692..715)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 agtccctatc agtgatagag atcccagtgg ctttaccaac agtacgggtt agagctagaa
       61 atagcaagtt aacctaaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
      121 ctttttttca cgaggcggac actgattgac acggtttgct agctgtacaa aaaagcaggc
      181 tttaaaggaa ccaattcagt cgactggatc cggtaccaag gtcgggcagg aagagggcct
      241 atttcccatg attccttcat atttgcatat acgatacaag gctgttagag agataattag
      301 aattaatttg actgtaaaca caaagatatt agtacaaaaa attgtgagcg gataacaatt
      361 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac
      421 cgtaacttga aagtaattgt gagcgctcac aattatatat cttgtggaaa ggacgaaaca
      481 ccgagtcgcg tgtagcgaag cagggttaga gctagaaata gcaagttaac ctaaggctag
      541 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttctagac ccagcaattg
      601 tgagcgctca caatttacaa agttggcact cactcgctat tcgaactaat tctaccgggt
      661 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
      721 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
      781 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
      841 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
      901 cactagtctc gtg
//
