LOCUS       sequence-159232-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the proteolysis-deficient mutant DLD-S456A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, 
            Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, 
            Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster
            LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson 
            J, Babu M
  TITLE     A Map of Human Mitochondrial Protein Interactions Linked to 
            Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and 
            NF-kappaB Signaling.
  JOURNAL   Cell Syst. 2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010.
  PUBMED    29128334
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010."
COMMENT     Sequence Label: pPHAGE_DLD-S456A-C-TAP
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(545..569)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             601..624
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             640..666
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             640..666
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(834..851)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 attgaactag atcccagagg tagaattcca gtcaatacca gatttcaaac taaaattcca
       61 aatatctatg ccattggtga tgtagttgct ggtccaatgc tggctcacaa agcagaggat
      121 gaaggcatta tctgtgttga aggaatggct ggtggtgctg tgcacattga ctacaattgt
      181 gtgccatcag tgatttacac acaccctgaa gttgcttggg ttggcaaatc agaagagcag
      241 ttgaaagaag agggtattga gtacaaagtt gggaaattcc catttgctgc taacagcaga
      301 gctaagacaa atgctgacac agatggcatg gtgaagatcc ttgggcagaa atcgacagac
      361 agagtactgg gagcacatat tcttggacca ggtgctggag aaatggtaaa tgaagctgct
      421 cttgctttgg aatatggagc atcctgtgaa gatatagcta gagtctgtca tgcacatccg
      481 accttagctg aagcttttag agaagcaaat cttgctgcgt catttggcaa atcaatcaac
      541 ttttgcccaa ctttcttgta caaagtggtt gatctagagg gcccgcggtt cgaactcgag
      601 gactacaagg acgacgatga caagtcggcc gctggaggat acccctacga cgtgcccgac
      661 tacgcctaga attccgcccc ccccccctaa cgttactggc cgaagccgct tggaataagg
      721 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
      781 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
      841 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
      901 a
//
