LOCUS       sequence-159233-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the proteolysis-deficient mutant DLD-S456A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, 
            Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, 
            Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster
            LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson 
            J, Babu M
  TITLE     A Map of Human Mitochondrial Protein Interactions Linked to 
            Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and 
            NF-kappaB Signaling.
  JOURNAL   Cell Syst. 2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010.
  PUBMED    29128334
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010."
COMMENT     Sequence Label: pPHAGE_DLD-S456A-C-TAP
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(821..845)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             877..900
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             916..942
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             916..942
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcagatgtga cagcagttga atttttaggt catgtaggtg gagttggaat tgatatggag
       61 atatctaaaa actttcaacg catccttcaa aaacaggggt ttaaatttaa attgaataca
      121 aaggttactg gtgctaccaa gaagtcagat ggaaaaattg atgtttctat tgaagctgct
      181 tctggtggta aagctgaagt tatcacttgt gatgtactct tggtttgcat tggccgacga
      241 ccctttacta agaatttggg actagaagag ctgggaattg aactagatcc cagaggtaga
      301 attccagtca ataccagatt tcaaactaaa attccaaata tctatgccat tggtgatgta
      361 gttgctggtc caatgctggc tcacaaagca gaggatgaag gcattatctg tgttgaagga
      421 atggctggtg gtgctgtgca cattgactac aattgtgtgc catcagtgat ttacacacac
      481 cctgaagttg cttgggttgg caaatcagaa gagcagttga aagaagaggg tattgagtac
      541 aaagttggga aattcccatt tgctgctaac agcagagcta agacaaatgc tgacacagat
      601 ggcatggtga agatccttgg gcagaaatcg acagacagag tactgggagc acatattctt
      661 ggaccaggtg ctggagaaat ggtaaatgaa gctgctcttg ctttggaata tggagcatcc
      721 tgtgaagata tagctagagt ctgtcatgca catccgacct tagctgaagc ttttagagaa
      781 gcaaatcttg ctgcgtcatt tggcaaatca atcaactttt gcccaacttt cttgtacaaa
      841 gtggttgatc tagagggccc gcggttcgaa ctcgaggact acaaggacga cgatgacaag
      901 tcggccgctg gaggataccc ctacgacgtg cccgactacg cctagaattc cgcccccccc
      961 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtt
//
