LOCUS       sequence-159234-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral construct for building stable cell lines expressing Cas9 
            in the presence of TNFa via puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Perli SD, Cui CH, Lu TK
  TITLE     Continuous genetic recording with self-targeting CRISPR-Cas in human
            cells.
  JOURNAL   Science. 2016 Aug 18. pii: aag0511.
  PUBMED    27540006
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 18. pii: aag0511."
COMMENT     Sequence Label: Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      261..270
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tccgaggttc tagacaggtg ccagaacatt tctctatcga taggtaccgg ggactttccg
       61 ggaatttccg gggactttcc gggaatttcc gggaatttcc ggggactttc cgggaatttc
      121 cggggacttt ccgggaattt ccagatctgg cctcggcggc caagcttgct agcggggggc
      181 tataaaaggg ggtgggggcg ttcgtcctca ctctagttct gcgatctaag taagcttggc
      241 attaccggtc gccaacgcgt gccaccatgg acaagaagta cagcatcggc ctggacatcg
      301 gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat
      361 tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc
      421 tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat
      481 acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca
      541 aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga
      601 agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt
      661 accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc
      721 ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg
      781 gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct
      841 acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgc
//
