LOCUS       sequence-159237-         545 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 70nt stgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Perli SD, Cui CH, Lu TK
  TITLE     Continuous genetic recording with self-targeting CRISPR-Cas in human
            cells.
  JOURNAL   Science. 2016 Aug 18. pii: aag0511.
  PUBMED    27540006
REFERENCE   2  (bases 1 to 545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 18. pii: aag0511."
COMMENT     Sequence Label: Construct 11 - U6p_70nt_stgRNA
FEATURES             Location/Qualifiers
     source          1..545
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     120..139
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(355..379)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(439..462)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt
       61 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
      121 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
      181 ggaaaggacg gaacaccgca aatacctcac acactcccaa tacatgaatc accacattat
      241 atcaattact tcttaaatca cacaatcagg gttagagcta gaaatagcaa gttaacctaa
      301 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt ctagacccag
      361 ctttcttgta caaagttggc attagacgtc gaggaattct accgggtagg ggaggcgctt
      421 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
      481 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
      541 tttgg
//
