LOCUS       sequence-159240-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon
            (for native or N-terminal fusions), G12D mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Esposito
  TITLE     RAS mutant collection V2.0
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Hs.NRAS G12D
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..124
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(716..815)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 aattcgagct cggtccgagg atccgaaata atgattttat tttgactgat agtgacctgt
       61 tcgttgcaac aaattgataa gcaatgcttt tttataatgc caactttgta caaaaaagtt
      121 ggcaggagag atggctctgc cgccaccatg actgagtaca aactggtggt ggttggagca
      181 gatggtgttg ggaaaagcgc actgacaatc cagctaatcc agaaccactt tgtagatgaa
      241 tatgatccca ccatagagga ttcttacaga aaacaagtgg ttatagatgg tgaaacctgt
      301 ttgttggaca tactggatac agctggacaa gaagagtaca gtgccatgag agaccaatac
      361 atgaggacag gcgaaggctt cctctgtgta tttgccatca ataatagcaa gtcatttgcg
      421 gatattaacc tctacaggga gcagattaag cgagtaaaag actcggatga tgtacctatg
      481 gtgctagtgg gaaacaagtg tgatttgcca acaaggacag ttgatacaaa acaagcccac
      541 gaactggcca agagttacgg gattccattc attgaaacct cagccaagac cagacagggt
      601 gttgaagatg ctttttacac actggtaaga gaaatacgcc agtaccgaat gaaaaaactc
      661 aacagcagtg atgatgggac tcagggttgt atgggattgc catgtgtggt gatgtaatca
      721 actttcttgt acaaagttgg cattatagga aagcattgct tatcaatttg ttgcaacgaa
      781 caggtcacta tcagtcaaaa taaaatcatt atttgcatgc aagcttagat cc
//
