LOCUS       sequence-159241-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mutation based toggling reporter (MTBR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Perli SD, Cui CH, Lu TK
  TITLE     Continuous genetic recording with self-targeting CRISPR-Cas in human
            cells.
  JOURNAL   Science. 2016 Aug 18. pii: aag0511.
  PUBMED    27540006
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 18. pii: aag0511."
COMMENT     Sequence Label: Construct 13 - CMVp_U6p_27nt1_GFP(+3)_RFP(+2)
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     117..136
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     CDS             join(373..375,376..888)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(415..436)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(676..695)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gagagataat ttgaatttat ttgactgtaa acacaaagat attagtacaa aatacgtgac
       61 gtcgaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt taaatggact
      121 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      181 aaggacgaaa caccgattca tctcatctat cagaaacaac agggttggag caagaaattg
      241 caagtcaacc taaggctagt ccgttatcaa cttgcaaaag tggcaccgag tcggtgcttt
      301 tttaccggaa gcggagctac tcacttcagc ctgctgaagc aggctggaga cgtggaggag
      361 aaccctggac ctgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      421 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      481 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      541 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
      601 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      661 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      721 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      781 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
      841 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
      901 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgcc
//
