LOCUS       sequence-159245-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 40nt stgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Perli SD, Cui CH, Lu TK
  TITLE     Continuous genetic recording with self-targeting CRISPR-Cas in human
            cells.
  JOURNAL   Science. 2016 Aug 18. pii: aag0511.
  PUBMED    27540006
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 18. pii: aag0511."
COMMENT     Sequence Label: Construct 10 - U6p_40nt_stgRNA
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     120..139
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(325..349)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(409..432)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     806..825
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt
       61 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
      121 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
      181 ggaaaggacg aaacaccgtc accacattat atcaattact tcttaaatca cacaatcagg
      241 gttagagcta gaaatagcaa gttaacctaa ggctagtccg ttatcaactt gaaaaagtgg
      301 caccgagtcg gtgctttttt ctagacccag ctttcttgta caaagttggc attagacgtc
      361 gaggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
      421 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
      481 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
      541 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
      601 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
      661 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
      721 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
      781 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
      841 ctgttctcct cttcctcatc tccggg
//
