LOCUS       sequence-159246-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses cytoplasmic restricted form of N-terminal myc tagged and 
            C-terminal flag tagged mRNA capping enzyme.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Otsuka Y, Kedersha NL, Schoenberg DR
  TITLE     Identification of a cytoplasmic complex that adds a cap onto 
            5'-monophosphate RNA.
  JOURNAL   Mol Cell Biol. 2009 Apr;29(8):2155-67. doi: 10.1128/MCB.01325-08. 
            Epub 2009 Feb 17.
  PUBMED    19223470
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01325-08"; journalName: "Mol Cell Biol"; date: 
            "2009-04"; volume: "29"; issue: "8"; pages: "2155-67"
COMMENT     Sequence Label: pcDNA4/TO-myc NES-mCE NLS-Flag
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..24)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttatcgtcg tcatccttgt aatcggttgg ccgatgcagt cttttgggcg gtgggggcgg
       61 catgagctcc gtgtcagggt ccaggggctg tccctgggcg gctgctgcac atctgtcaat
      121 gaattcaaac agcatctcct tggtgacagg gttcgagatg ctattgcaca cagccatggc
      181 tgtgttgtag gcatttggga aacttttgtc tatcctctgt ctcatgaaga cccagctatt
      241 gttctcaaat ttgcattcta taattttgtt gtcatactgt tttagttctt ttgtcacctt
      301 gatttgtgca aagggtcttt cataacctcc aacatagaga aggccaacat tctgtggaag
      361 caacccttct cctcccattc ttgttatctt aagtcgaaaa tccacagagt ttagactggg
      421 aggtttccat ttcaaaatgt catcacatcg accaggcttg tattttccaa taggctgaaa
      481 aataagtcca tccatttcat ggctgacttc tttggcaaaa tttccttcca gaagctttct
      541 tgaaatattg atgtcaaaaa attgtttagg tctgacacta aatggttcct gtgttttgtc
      601 aatgagtcca gtcttcattt tttcatgtcg tggacttata atttcacgtt caatacactg
      661 tagacgaata ttaaaatcac agtctccaac tggttgtgca ttgaacttaa ttatgtcata
      721 gatcaaatat cttgggacgg cctgtccatt tactttgtca atgatcattt ccccatccaa
      781 aagagtattt gataaatgca tgcgaagatc tttacgaaat ggaaattcca gatttgaaac
      841 atgaaaaacg gaattatctc tgtcaatcat aaaaacttca tttgtgccat caatcaacat
      901 catgtaacga gtgccgtctg ctttccagct tactttataa ggcttctgct ctaaaagtct
      961 aatattttgc ttgtccatgg agacagg
//
