LOCUS       sequence-159247-         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For assaying self-targeting activity via Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Perli SD, Cui CH, Lu TK
  TITLE     Continuous genetic recording with self-targeting CRISPR-Cas in human
            cells.
  JOURNAL   Science. 2016 Aug 18. pii: aag0511.
  PUBMED    27540006
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 18. pii: aag0511."
COMMENT     Sequence Label: Construct 3 - U6p_20nt1_mod2_sgRNA (stgRNA)
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(106..354)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(164..183)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(334..354)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    496..613
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tagaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta gccttaggtt
       61 aacttgctat ttctagctct aacccaggac agtactccga cttacggtgt ttcgtccttt
      121 ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa gcatatgata
      181 gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt actttctacg
      241 tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattctaa ttatctctct
      301 aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc cctcttcctg
      361 cccgaccttg gtaccggatc cagtcgactg aattggttcc tttaaagcct gcttttttgt
      421 acaaggtggc acttttcggg gaaatgtgcg cggaaccccc caaactggat ctctgctgtc
      481 cctgtaataa acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg
      541 tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc
      601 ttttctttta aaattgtgga tgaatactgc c
//
