LOCUS       sequence-159249-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize
            using Asc1 and Not1 for mouse embryo injection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Mohr MA, Argast P, Pantazis P
  TITLE     Labeling cellular structures in vivo using confined primed 
            conversion of photoconvertible fluorescent proteins.
  JOURNAL   Nat Protoc. 2016 Dec;11(12):2419-2431. doi: 10.1038/nprot.2016.134. 
            Epub 2016 Nov 3.
  PUBMED    27809312
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2016.134"; journalName: "Nat Protoc"; date: 
            "2016-12"; volume: "11"; issue: "12"; pages: "2419-2431"
COMMENT     Sequence Label: p55-H2B-Dendra2
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     250..272
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     264..281
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     265..281
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    353..577
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     566..583
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
       61 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
      121 atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
      181 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
      241 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tggcggccgc tctagcctcg
      301 aggctagaac tagtggatct cgagccccag ctggttcttt ccgcctcaga agccatagag
      361 cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc
      421 cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca
      481 ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag
      541 gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgagctcga
      601 cgtctcactt gtacacgccg ctgtcgccgg accacacctg gctgggcagg gggctgtagc
      661 gggccacggc gtgctcgtac agcttcacct tgttgtagtc gctgtcgttg cccaggatct
      721 cgatgcggtg gtccacgaag tgggcgtcgg gcagctgcac caccttcttg gccttgtagg
      781 tggtcttgaa gtcgcacagg tagtggccgc cgccctccag cagcagggcc atgttgatgt
      841 tgcccaccag caggccgtcg cgcacgtgca gcttctcggt gctgggctcc cacttcaggg
      901 tcttcttctg catcacgggg ccgttggggg gg
//
