LOCUS       sequence-159252-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Replaces #61376. QUAS driving GFP with beta crystalline CFP eye 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  TITLE     QUAS:GFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Quas:GFPpA;Betacrystallin:CFP
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    626..760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(663..682)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     717..736
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     776..792
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    800..820
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(894..915)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tacttgagta attttacttg attactgtac ttaagtatta tttttgggga tttttacttt
       61 acttgagtac aattaaaaat caatactttt acttttactt aattacattt ttttagaaaa
      121 aaaagtactt tttactcctt acaattttat ttacagtcaa aaagtactta ttttttggag
      181 atcacttcat tctattttcc cttgctatta ccaaaccaat tgaattgcgc tgatgcccag
      241 tttaatttaa atgttattta ttctgcctat gaaaatcgtt ttcacattat atgaaattgg
      301 tcagacatgt tcattggtcc tttggaagtg acgtcatgtc acatctatta ccacaatgca
      361 cagcaccttg acctggaaat tagggaaatt ataacagtca atcagtggaa gaaaatggag
      421 gaagtatgtg attcatcagc agctgcgagc agcacagtcc aaaatcagcc acaggatcaa
      481 gagcacccgt ggccgtatct tcgcagatct gatctagagg atcataatca gccataccac
      541 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
      601 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
      661 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
      721 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc atcatcgatg gtaccgtaaa
      781 acgacggcca gtgaattatc aactatgtat aataaagttg tcgaggctga tcagcgggtt
      841 tggccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
      901 tccagcagga ccatgtgatc gcgct
//
