LOCUS       sequence-159254-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Replaces #61376. QUAS driving GFP with beta crystalline CFP eye 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  TITLE     QUAS:GFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Quas:GFPpA;Betacrystallin:CFP
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     90..106
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     90..106
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    126..146
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    337..361
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(412..433)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(673..692)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tattagtctt gatagagagg ctgcaaatag caggaaacgt gagcagagac tccctggtgt
       61 ctgaaacaca ggccagatgg gccctcgagc aggaaacagc tatgaccatg attacgccaa
      121 gctatcaact ttgtatagaa aagttggggt aatcgcttat cctcggataa acaattatcc
      181 tcacgggtaa tcgcttatcc gctcgggtaa tcgcttatcc tcgggtaatc gcttatcctt
      241 gcaagggtcg actctagagg gtatataatg gatcccatcg cgtctcagcc tcactttgag
      301 ctcctccaca cgaattccct cgacctcgaa gacgcgtcaa gtttgtacaa aaaagcaggc
      361 tccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      421 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      481 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      541 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
      601 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      661 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      721 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      781 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatgg
//
