LOCUS       sequence-159261-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses monomeric streptavidin (mSA) in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Chamma I, Letellier M, Butler C, Tessier B, Lim KH, Gauthereau I, 
            Choquet D, Sibarita JB, Park S, Sainlos M, Thoumine O
  TITLE     Mapping the dynamics and nanoscale organization of synaptic adhesion
            proteins using monomeric streptavidin.
  JOURNAL   Nat Commun. 2016 Mar 16;7:10773. doi: 10.1038/ncomms10773.
  PUBMED    26979420
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-16"; volume: "7:10773. doi"; pages: " 
            10.1038/ncomms10773"
COMMENT     Sequence Label: pET-IG-mSA
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(104..151)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     147..165
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(218..235)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(260..601)
                     /codon_start=1
                     /product="monomeric streptavidin/rhizavidin hybrid (Lim et 
                     al., 2013)"
                     /label=mSA
                     /translation="AEAGITGTWYNQSGSTFTVTAGADGNLTGQYENRAQGTGCQNSPY
                     TLTGRYNGTKLEWRVEWNNSTENCHSRTEWRGQYQGGAEARINTQWNLTYEGGSGPATE
                     QGQDTFTKVK"
     CDS             complement(608..628)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(629..658)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     RBS             675..697
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    712..736
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(736..755)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(737..755)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(839..858)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
       61 ggcgcgtccc attcgccaat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
      121 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
      181 cagcttcctt tcgggctttg ttagcagccg gatctcagtg gtggtggtgg tggtgctcga
      241 gttaagacgc cgcagacggt ttaactttgg tgaaggtgtc ctgaccctgt tcggtcgccg
      301 gaccagaacc accttcgtag gtcaggttcc actgggtgtt gatacgcgct tccgcaccac
      361 cctggtactg accacgccat tcggtacgag agtggcagtt ttcggtagag ttgttccatt
      421 caacacgcca ttccagtttg gtaccgttgt aacgaccggt cagggtgtac ggagagttct
      481 ggcaaccagt gccctgcgca cggttttcgt actgaccggt caggttaccg tccgcacccg
      541 cggtaacggt gaaggtagaa ccagactggt tgtaccacgt gccggtgata cccgcttccg
      601 cggatccgct ttgaaaatac agattttcgt gatggtgatg atgatgatgg tgatggtggc
      661 tagccatatg tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt
      721 atccgctcac aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc
      781 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
      841 tatatcgccg acatcaccga tgggga
//
