LOCUS       sequence-159264-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express human caspase 8 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Oberst A, Pop C, Tremblay AG, Blais V, Denault JB, Salvesen GS, 
            Green DR
  TITLE     Inducible dimerization and inducible cleavage reveal a requirement 
            for both processes in caspase-8 activation.
  JOURNAL   J Biol Chem. 2010 May 28;285(22):16632-42. doi: 
            10.1074/jbc.M109.095083. Epub 2010 Mar 22.
  PUBMED    20308068
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.095083"; journalName: "J Biol Chem"; date: 
            "2010-05-28- 28"; volume: "285"; issue: "22"; pages: "16632-42"
COMMENT     Sequence Label: Fv-hCaspase 8-2A-GFP
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..52
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             59..379
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 cggatctacc atggggagta gcaagagcaa gcctaaggac cccagccagc gctctagagg
       61 agtgcaggtg gagactatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac
      121 ctgcgtggtg cactacaccg ggatgcttga agatggaaag aaagttgatt cctcccggga
      181 cagaaacaag ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga
      241 aggggttgcc cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc
      301 ctatggtgcc actgggcacc caggcatcat cccaccacat gccactctcg tcttcgatgt
      361 ggagcttcta aaactggaaa ctagtggagg tggaggttct agaggatccc atatgacaat
      421 ctcggactct ccaagagaac aggatagtga atcacagact ttggacaaag tttaccaaat
      481 gaaaagcaaa cctcggggat actgtctgat catcaacaat cacaattttg caaaagcacg
      541 ggagaaagtg cccaaacttc acagcattag ggacaggaat ggaacacact tggatgcagg
      601 ggctttgacc acgacctttg aagagcttca ttttgagatc aagccccacg atgactgcac
      661 agtagagcaa atctatgaga ttttgaaaat ctaccaactc atggaccaca gtaacatgga
      721 ctgcttcatc tgctgtatcc tctcccatgg agacaagggc atcatctatg gcactgatgg
      781 acaggaggcc cccatctatg agctgacatc tcagttcact ggtttgaagt gcccttccct
      841 tgctggaaaa cccaaagtgt tttttattca ggcttgtcag ggggataact accagaaagg
      901 tatacctgtt gagactgatt cagaggagca accctattta gaaatggatt tatcatcacc
      961 tcaaacgaga tatatcccgg atgaggctga ctttctgctg gggatggcca ctgtgaataa
//
