LOCUS       sequence-159266-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses cytoplasmic restricted form of N-terminal myc tagged and 
            C-terminal flag tagged mRNA capping enzyme, inactive form.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Otsuka Y, Kedersha NL, Schoenberg DR
  TITLE     Identification of a cytoplasmic complex that adds a cap onto 
            5'-monophosphate RNA.
  JOURNAL   Mol Cell Biol. 2009 Apr;29(8):2155-67. doi: 10.1128/MCB.01325-08. 
            Epub 2009 Feb 17.
  PUBMED    19223470
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01325-08"; journalName: "Mol Cell Biol"; date: 
            "2009-04"; volume: "29"; issue: "8"; pages: "2155-67"
COMMENT     Sequence Label: pcDNA4/TO-myc  NES-mCE(K294A) NLS-Flag
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..32)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggcccctact tatcgtcgtc atccttgtaa tcggttggcc gatgcagtct tttgggcggt
       61 gggggcggca tgagctccgt gtcagggtcc aggggctgtc cctgggcggc tgctgcacat
      121 ctgtcaatga attcaaacag catctccttg gtgacagggt tcgagatgct attgcacaca
      181 gccatggctg tgttgtaggc atttgggaaa cttttgtcta tcctctgtct catgaagacc
      241 cagctattgt tctcaaattt gcattctata attttgttgt catactgttt tagttctttt
      301 gtcaccttga tttgtgcaaa gggtctttca taacctccaa catagagaag gccaacattc
      361 tgtggaagca acccttctcc tcccattctt gttatcttaa gtcgaaaatc cacagagttt
      421 agactgggag gtttccattt caaaatgtca tcacatcgac caggcttgta ttttccaata
      481 ggctgaaaaa taagtccatc catttcatgg ctgacttctt tggcaaaatt tccttccaga
      541 agctttcttg aaatattgat gtcaaaaaat tgtttaggtc tgacactaaa tggttcctgt
      601 gttttgtcaa tgagtccagt cttcattttt tcatgtcgtg gacttataat ttcacgttca
      661 atacactgta gacgaatatt aaaatcacag tctccaactg gttgtgcatt gaacttaatt
      721 atgtcataga tcaaatatct tgggacggcc tgtccattta ctttgtcaat gatcatttcc
      781 ccatccaaaa gagtatttga taaatgcatg cgaagatctt tacgaaatgg aaattccaga
      841 tttgaaacat gaaaaacgga attatctctg tcaatcataa aaacttcatt tgtgccatca
      901 atcaacatca tgtaacgagt gccgtctgct gcccagctta ctttataagg cttctgctct
      961 aaaagtctaa tattttgctt gtccatggag acaggctgtg ctcc
//
