LOCUS       sequence-159267-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express human caspase 8 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Oberst A, Pop C, Tremblay AG, Blais V, Denault JB, Salvesen GS, 
            Green DR
  TITLE     Inducible dimerization and inducible cleavage reveal a requirement 
            for both processes in caspase-8 activation.
  JOURNAL   J Biol Chem. 2010 May 28;285(22):16632-42. doi: 
            10.1074/jbc.M109.095083. Epub 2010 Mar 22.
  PUBMED    20308068
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.095083"; journalName: "J Biol Chem"; date: 
            "2010-05-28- 28"; volume: "285"; issue: "22"; pages: "16632-42"
COMMENT     Sequence Label: Fv-hCaspase 8-2A-GFP
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(6..719,720..722,723..725))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(9..719,720..722,723..725))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(51..72)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     400..419
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     659..680
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(735..788)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ttaacttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
       61 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc
      121 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
      181 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg
      241 atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc
      301 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
      361 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
      421 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
      481 tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc
      541 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc
      601 ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg
      661 tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc
      721 accataggcg cgccagggcc gggattctcc tccacgtcac cgcatgttag aagacttcct
      781 ctgccctctg cgatcgcatc agaagggaag acaagttttt ttcttagtgt gaaagtaggc
      841 tgaggcatct gtttccccat gtttttcttg tcatccttgt tgcttacttc atagttcact
      901 tcagtcagga tggtgagaat atcatcgcct cgaggacatc gctctctcag gctctggcaa
      961 agtgactgga tgtaccaggt tccctctgca gggtttcggt aggaaacaca gttattc
//
