LOCUS       sequence-159270-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of MK14. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: MK14_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             58..80
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..129
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             130..150
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tccggagagc tctttaatta agcggccgcc ctgcaggact cgagttctag aaataatttt
       61 gtttaacttt aagaaggaga tatacatatg aaatcttctc accatcacca tcaccatcac
      121 catcaccatg aaaacctgta cttccaatcc aatgcaatgt ctcaggagag gcccacgttc
      181 taccggcagg agctgaacaa gacaatctgg gaggtgcccg agcgttacca gaacctgtct
      241 ccagtgggct ctggcgccta tggctctgtg tgtgctgctt ttgacacaaa aacggggtta
      301 cgtgtggcag tgaagaagct ctccagacca tttcagtcca tcattcatgc gaaaagaacc
      361 tacagagaac tgcggttact taaacatatg aaacatgaaa atgtgattgg tctgttggac
      421 gtttttacac ctgcaaggtc tctggaggaa ttcaatgatg tgtatctggt gacccatctc
      481 atgggggcag atctgaacaa cattgtgaaa tgtcagaagc ttacagatga ccatgttcag
      541 ttccttatct accaaattct ccgaggtcta aagtatatac attcagctga cataattcac
      601 agggacctaa aacctagtaa tctagctgtg aatgaagact gtgagctgaa gattctggat
      661 tttggactgg ctcggcacac agatgatgaa atgacaggct acgtggccac taggtggtac
      721 agggctcctg agatcatgct gaactggatg cattacaacc agacagttga tatttggtca
      781 gtgggatgca taatggccga gctgttgact ggaagaacat tgtttcctgg tacagaccat
      841 attgatcagt tgaagctcat tttaagactc gttgga
//
