LOCUS       sequence-159271-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of MK14. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: MK14_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             186..194
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tttcgggctt tgttagcagc cggatcagcg cgcacgcgtg gtaccggccg gccaccaggt
       61 ggcgcgccgc gatcgcggat ccgttatcca cttccaatgt tattaggact ccatctcttc
      121 ttggtcaagg ggtggtggca caaagctgat gacttcatca taggtcaggc ttttccactc
      181 atctataagg aggtccctgc tttcaaagga ctgatcataa ggatcggcca ctggttcatc
      241 atcaggatcg tggtactgag caaagtaggc atgtgcaagg gcttgggccg ctgtaattct
      301 cttatctgag tccaatacaa gcatcttctc cagcaagtcg acagccaggg gattggcacc
      361 aataaataca ttcgcaaagt tcatcttcgg catctgagtc aaagactgaa tatagtttct
      421 tgcagactct gaggagattt tcttcaaaag ctcagcccct ggggttccaa cgagtcttaa
      481 aatgagcttc aactgatcaa tatggtctgt accaggaaac aatgttcttc cagtcaacag
      541 ctcggccatt atgcatccca ctgaccaaat atcaactgtc tggttgtaat gcatccagtt
      601 cagcatgatc tcaggagccc tgtaccacct agtggccacg tagcctgtca tttcatcatc
      661 tgtgtgccga gccagtccaa aatccagaat cttcagctca cagtcttcat tcacagctag
      721 attactaggt tttaggtccc tgtgaattat gtcagctgaa tgtatatact ttagacctcg
      781 gagaatttgg tagataagga actgaacatg gtcatctgta agcttctgac atttcacaat
      841 gttgttcaga tctgccccca tgagatgggt caccagatac acatcattga attcctccag
      901 agaccttgca ggtgtaaaaa cgtccaacag accaatcaca ttttcatgtt tcatatgttt
      961 aagtaaccgc agttctctgt aggttctttt cgcatgaatg atggactgaa atggtctgga
     1021 gagcttcttc actgccac
//
