LOCUS       sequence-159277-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of VRK1. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: VRK1_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             58..80
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..129
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             130..150
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tccggagagc tctttaatta agcggccgcc ctgcaggact cgagttctag aaataatttt
       61 gtttaacttt aagaaggaga tatacatatg aaatcttctc accatcacca tcaccatcac
      121 catcaccatg aaaacctgta cttccaatcc aatgcacgtg taaaagcagc tcaagctgga
      181 agacagagct ctgcaaagag acatcttgca gaacaatttg cagttggaga gataataact
      241 gacatggcaa aaaaggaatg gaaagtagga ttacccattg gccaaggagg ctttggctgt
      301 atatatcttg ctgatatgaa ttcttcagag tcagttggca gtgatgcacc ttgtgttgta
      361 aaagtggaac ccagtgacaa tggacctctt tttactgaat taaagttcta ccaacgagct
      421 gcaaaaccag agcaaattca gaaatggatt cgtacccgta agctgaagta cctgggtgtt
      481 cctaagtatt gggggtctgg tctacatgac aaaaatggaa aaagttacag gtttatgata
      541 atggatcgct ttgggagtga ccttcagaaa atatatgaag caaatgccaa aaggttttct
      601 cggaaaactg tcttgcagct aagcttaaga attctggata ttctggaata tattcacgag
      661 catgagtatg tgcatggaga tatcaaggcc tcaaatcttc ttctgaacta caagaatcct
      721 gaccaggtgt acttggtaga ttatggcctt gcttatcggt actgcccaga aggagttcat
      781 aaagaataca aagaagaccc caaaagatgt cacgatggca ctattgaatt cacgagcatc
      841 gatgcacaca atggcgtggc cccatcaaga cgtggtgatt tggaaatact tggttattgc
      901 atgatccaat ggcttactgg ccatcttcct tgggaggata atttgaaaga tcctaa
//
