LOCUS       sequence-159278-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of VRK1. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: VRK1_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttcgggctt tgttagcagc cggatcagcg cgcacgcgtg gtaccggccg gccaccaggt
       61 ggcgcgccgc gatcgcggat ccgttatcca cttccaatgt tattattctt caatttcttt
      121 ctttcgcttc tttgttattg tttttgcttt caaacctcca ttctccacaa cactgaggtc
      181 caatttgcca tcatccttac ttcctatagc ttttagtcct tgcaaaagaa tgtcacgtaa
      241 attttcataa agaggttttt cagtgtagtc tagtaatttc actgtttcca tgtatttggc
      301 aatttcacct ggtttgtttt tctcaggaaa acatttgtcc atcaaacttg caatattttc
      361 tctgtatcta attttggaat ctctaacata tttaggatct ttcaaattat cctcccaagg
      421 aagatggcca gtaagccatt ggatcatgca ataaccaagt atttccaaat caccacgtct
      481 tgatggggcc acgccattgt gtgcatcgat gctcgtgaat tcaatagtgc catcgtgaca
      541 tcttttgggg tcttctttgt attctttatg aactccttct gggcagtacc gataagcaag
      601 gccataatct accaagtaca cctggtcagg attcttgtag ttcagaagaa gatttgaggc
      661 cttgatatct ccatgcacat actcatgctc gtgaatatat tccagaatat ccagaattct
      721 taagcttagc tgcaagacag ttttccgaga aaaccttttg gcatttgctt catatatttt
      781 ctgaaggtca ctcccaaagc gatccattat cataaacctg taactttttc catttttgtc
      841 atgtagacca gacccccaat acttaggaac acccaggtac ttcagcttac gggtacgaat
      901 ccatttctga atttgctctg gttttgcagc tcgttggtag aactttaatt cagtaaaaag
      961 aggtccattg tcactgggtt ccacttttac aacacaaggt gcatcactgc caactgactc
     1021 tgaagaattc atatcagcaa ga
//
