LOCUS       sequence-159279-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of PAK6. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: PAK6_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             61..83
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             103..132
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             133..153
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggctccggag agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat
       61 tttgtttaac tttaagaagg agatatacat atgaaatctt ctcaccatca ccatcaccat
      121 caccatcacc atgaaaacct gtacttccaa tccaatgcag gtgttgtgac acatgagcag
      181 ttcaaggctg cgctcaggat ggtggtggac cagggtgacc cccggctgct gctggacagc
      241 tacgtgaaga ttggcgaggg ctccaccggc atcgtctgct tggcccggga gaagcactcg
      301 ggccgccagg tggccgtcaa gatgatggac ctcaggaagc agcagcgcag ggagctgctc
      361 ttcaacgagg tggtgatcat gcgggactac cagcacttca acgtggtgga gatgtacaag
      421 agctacctgg tgggcgagga gctgtgggtg ctcatggagt tcctgcaggg aggagccctc
      481 acagacatcg tctcccaagt caggctgaat gaggagcaga ttgccactgt gtgtgaggct
      541 gtgctgcagg ccctggccta cctgcatgct cagggtgtca tccaccggga catcaagagt
      601 gactccatcc tgctgaccct cgatggcagg gtgaagctct cggacttcgg attctgtgct
      661 cagatcagca aagacgtccc taagaggaag tccctggtgg gaacccccta ctggatggct
      721 cctgaagtga tctccaggtc tttgtatgcc actgaggtgg atatctggtc tctgggcatc
      781 atggtgattg agatggtaga tggggagcca ccgtacttca gtgactcccc agtgcaagcc
      841 atgaagaggc tccgggacag ccccccaccc aagctgaaaa actctcacaa ggtctcccca
      901 gtgctgcgag acttcctgga gcggatgctg gtgcgggacc cccaagagag agccacagcc
      961 caggagct
//
