LOCUS       sequence-159280-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of PAK6. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: PAK6_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1003..1023)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(1024..1050)
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
ORIGIN
        1 ctttgttagc agccggatca gcgcgcacgc gtggtaccgg ccggccacca ggtggcgcgc
       61 cgcgatcgcg gatccgttat ccacttccaa tgttattagc aggtggaggt ctgctttcgg
      121 tagagctgga tcaggggcac caggcactca ggtagccctg tctgcagcag gaaggggtgg
      181 tctaggagct cctgggctgt ggctctctct tgggggtccc gcaccagcat ccgctccagg
      241 aagtctcgca gcactgggga gaccttgtga gagtttttca gcttgggtgg ggggctgtcc
      301 cggagcctct tcatggcttg cactggggag tcactgaagt acggtggctc cccatctacc
      361 atctcaatca ccatgatgcc cagagaccag atatccacct cagtggcata caaagacctg
      421 gagatcactt caggagccat ccagtagggg gttcccacca gggacttcct cttagggacg
      481 tctttgctga tctgagcaca gaatccgaag tccgagagct tcaccctgcc atcgagggtc
      541 agcaggatgg agtcactctt gatgtcccgg tggatgacac cctgagcatg caggtaggcc
      601 agggcctgca gcacagcctc acacacagtg gcaatctgct cctcattcag cctgacttgg
      661 gagacgatgt ctgtgagggc tcctccctgc aggaactcca tgagcaccca cagctcctcg
      721 cccaccaggt agctcttgta catctccacc acgttgaagt gctggtagtc ccgcatgatc
      781 accacctcgt tgaagagcag ctccctgcgc tgctgcttcc tgaggtccat catcttgacg
      841 gccacctggc ggcccgagtg cttctcccgg gccaagcaga cgatgccggt ggagccctcg
      901 ccaatcttca cgtagctgtc cagcagcagc cgggggtcac cctggtccac caccatcctg
      961 agcgcagcct tgaactgctc atgtgtcaca acacctgcat ttggattgga agtacaggtt
     1021 ttcatggtga tggtgatggt gatggtgatg gttaaaa
//
