LOCUS       sequence-159282-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of FES. Intended for 
            co-expression with YopH Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: FES_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             49..71
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             91..120
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             121..141
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctctttaatt aagcggccgc cctgcaggac tcgagttcta gaaataattt tgtttaactt
       61 taagaaggag atatacatat gaaatcttct caccatcacc atcaccatca ccatcaccat
      121 gaaaacctgt acttccaatc caatgcaatt ccggaggtgc agaagcccct gcatgagcag
      181 ctgtggtacc acggggccat cccgagggca gaggtggctg agctgctggt gcactctggg
      241 gacttcctgg tgcgggagag ccagggcaag caggagtacg tgctgtcggt gctgtgggat
      301 ggtctgcccc ggcacttcat catccagtcc ttggataacc tgtaccgact ggaaggggaa
      361 ggctttccta gcattccttt gctcatcgac cacctactga gcacccagca gcccctcacc
      421 aagaagagtg gtgttgtcct gcacagggct gtgcccaagg acaagtgggt gctgaaccat
      481 gaggacctgg tgttgggtga gcagattgga cgggggaact ttggcgaagt gttcagcgga
      541 cgcctgcgag ccgacaacac cctggtggcg gtgaagtctt gtcgagagac gctcccacct
      601 gacctcaagg ccaagtttct acaggaagcg aggatcctga agcagtacag ccaccccaac
      661 atcgtgcgtc tcattggtgt ctgcacccag aagcagccca tctacatcgt catggagctt
      721 gtgcaggggg gcgacttcct gaccttcctc cgcacggagg gggcccgcct gcgggtgaag
      781 actctgctgc agatggtggg ggatgcagct gctggcatgg agtacctgga gagcaagtgc
      841 tgcatccacc gggacctggc tgctcggaac tgcctggtga cagagaagaa tgtcctgaag
      901 atcagtgact ttgggatgtc ccgagaggaa gccgatgggg tctatgcagc ctcagggggc
      961 ctcagacaag tccccgtgaa gtggaccgca cctgaggccc ttaactacgg ccgctactcc
     1021 tccgaaagcg acgtgtggag ctttggc
//
