LOCUS       sequence-159286-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of DMPK. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: DMPK_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             101..130
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             131..151
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     RBS             310..318
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ctccggagag ctctttaatt aagcggccgc cctgcaggac tcgagttcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat gaaatcttct caccatcacc atcaccatca
      121 ccatcaccat gaaaacctgt acttccaatc caatgcacag cagctggtgt tggacccggg
      181 cttcctgggg ctggagcccc tgctcgacct tctcctgggc gtccaccagg agctgggcgc
      241 ctccgaactg gcccaggaca agtacgtggc cgacttcttg cagtgggcgg agcccatcgt
      301 ggtgaggctt aaggaggtcc gactgcagag ggacgacttc gagattctga aggtgatcgg
      361 acgcggggcg ttcagcgagg tagcggtagt gaagatgaag cagacgggcc aggtgtatgc
      421 catgaagatc atgaacaagt gggacatgct gaagaggggc gaggtgtcgt gcttccgtga
      481 ggagagggac gtgttggtga atggggaccg gcggtggatc acgcagctgc acttcgcctt
      541 ccaggatgag aactacctgt acctggtcat ggagtattac gtgggcgggg acctgctgac
      601 actgctgagc aagtttgggg agcggattcc ggccgagatg gcgcgcttct acctggcgga
      661 gattgtcatg gccatagact cggtgcaccg gcttggctac gtgcacaggg acatcaaacc
      721 cgacaacatc ctgctggacc gctgtggcca catccgcctg gccgacttcg gctcttgcct
      781 caagctgcgg gcagatggaa cggtgcggtc gctggtggct gtgggcaccc cagactacct
      841 gtcccccgag atcctgcagg ctgtgggcgg tgggcctggg acaggcagct acgggcccga
      901 gtgtgactgg tgggcgctgg gtgtattcgc ctatgaaatg ttctatgg
//
