LOCUS       sequence-159288-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of PHKG2. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: PHKG2_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             58..80
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..129
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             130..150
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tccggagagc tctttaatta agcggccgcc ctgcaggact cgagttctag aaataatttt
       61 gtttaacttt aagaaggaga tatacatatg aaatcttctc accatcacca tcaccatcac
      121 catcaccatg aaaacctgta cttccaatcc aatgcagggc cggaggatga gctgcccgac
      181 tgggccgccg ccaaagagtt ttaccagaag tacgacccta aggacgtcat cggcagagga
      241 gtgagctctg tggtccgccg ttgtgttcat cgagctactg gccacgagtt tgcggtgaag
      301 attatggaag tgacagctga gcggctgagt cctgagcagc tggaggaggt gcgggaagcc
      361 acacggcgag agacacacat ccttcgccag gtcgccggcc acccccacat catcaccctc
      421 atcgattcct acgagtcttc tagcttcatg ttcctggtgt ttgacctgat gcggaaggga
      481 gagctgtttg actatctcac agagaaggtg gccctctctg aaaaggaaac caggtccatc
      541 atgcggtctc tgctggaagc agtgagcttt ctccatgcca acaacattgt gcatcgagat
      601 ctgaagcccg agaatattct cctagatgac aatatgcaga tccgactttc agatttcggg
      661 ttctcctgcc acttggaacc tggcgagaag cttcgagagt tgtgtgggac cccagggtat
      721 ctagcgccag agatccttaa atgctccatg gatgaaaccc acccaggcta tggcaaggag
      781 gtcgacctct gggcctgtgg ggtgatcttg ttcacactcc tggctggctc gccacccttc
      841 tggcaccggc ggcagatcct gatgttacgc atgatcatgg agggccagta ccagttcagt
      901 tcccccgagt gggatgaccg ttccagcact gtcaaagacc tgatctccag gctgctgcag
      961 gtggatcctg aggcacgcct gacagctgag caggccctac agca
//
