LOCUS       sequence-159289-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of PHKG2. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: PHKG2_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(968..988)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tttgttagca gccggatcag cgcgcacgcg tggtaccggc cggccaccag gtggcgcgcc
       61 gcgatcgcgg atccgttatc cacttccaat gttattaacg ctcaaagaag gggtgctgta
      121 gggcctgctc agctgtcagg cgtgcctcag gatccacctg cagcagcctg gagatcaggt
      181 ctttgacagt gctggaacgg tcatcccact cgggggaact gaactggtac tggccctcca
      241 tgatcatgcg taacatcagg atctgccgcc ggtgccagaa gggtggcgag ccagccagga
      301 gtgtgaacaa gatcacccca caggcccaga ggtcgacctc cttgccatag cctgggtggg
      361 tttcatccat ggagcattta aggatctctg gcgctagata ccctggggtc ccacacaact
      421 ctcgaagctt ctcgccaggt tccaagtggc aggagaaccc gaaatctgaa agtcggatct
      481 gcatattgtc atctaggaga atattctcgg gcttcagatc tcgatgcaca atgttgttgg
      541 catggagaaa gctcactgct tccagcagag accgcatgat ggacctggtt tccttttcag
      601 agagggccac cttctctgtg agatagtcaa acagctctcc cttccgcatc aggtcaaaca
      661 ccaggaacat gaagctagaa gactcgtagg aatcgatgag ggtgatgatg tgggggtggc
      721 cggcgacctg gcgaaggatg tgtgtctctc gccgtgtggc ttcccgcacc tcctccagct
      781 gctcaggact cagccgctca gctgtcactt ccataatctt caccgcaaac tcgtggccag
      841 tagctcgatg aacacaacgg cggaccacag agctcactcc tctgccgatg acgtccttag
      901 ggtcgtactt ctggtaaaac tctttggcgg cggcccagtc gggcagctca tcctccggcc
      961 ctgcattgga ttggaagtac aggttttcat ggtg
//
