LOCUS       sequence-159291-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of CSK. Intended for 
            co-expression with YopH Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: CSK_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(900..920)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(921..950)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     RBS             970..992
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ctttgttagc agccggatca gcgcgcacgc gtggtaccgg ccggccacca ggcggcgcgc
       61 cgcgatcgcg gatccgttat ccacttccaa tgttattaca ggtgcagctc gtgggttttg
      121 atgtgctcaa gctgctctcg gagctgtagg aaggagggcc gcatggcggc gtccaggtgc
      181 cagcagttct tcatgacttc atagactgcg ggcgggcagc cgtcgggggc atccatcttg
      241 tagcccttct ccacccgagg gacgacgtcc ttcaggggaa ttcttggata aggcactcgc
      301 ccaaaggagt agatttccca gagaaggatt ccgaaactcc acacgtcaga cttagtggag
      361 aatttcttct ctctcagggc ctcaggggct gtccacttga ctggcagctt gcccgtgtcc
      421 tgggtgctgg acgcctcctt ggtgagacca aagtcgctga ccttggccac attgtcctca
      481 gacaccagca cattgcgggc agccaggtct cgatgcacga aattgttgcc ctccaggtat
      541 tccatggcct cgcagacatc tagcgagaac ttgaggagac agtctccgcc cagcactgac
      601 cgacccctag accgcaggta gtccacaagg ctccccttgg ccatgtactc agtgacgatg
      661 tagagcccgc ccttctcctc cacgatcacg cccaggagct gcaccaggtt gctatgccgc
      721 agttgcgtca tgactgaggc ttcagccagg aaggcctggg cagtggcgtc gttcttaatg
      781 cacttgacgg cgactttgtt ccctcggtaa tcgcccagca tcacgtctcc gaactccccc
      841 ttcccgatgg tctgcagcag cttcagctcc ttcatgttca gggcccagcc gcttgcattg
      901 gattggaagt acaggttttc atggtgatgg tgatggtgat ggtgatggtg agaagatttc
      961 atatgtatat ctccttctta aagttaaaca aaattatttc tagaactcga gtcctgcagg
     1021 gcggccgc
//
